Fig. 1

Effect of increasing concentrations of OTR4131 on HSPC proliferation and viability. a–d HSPC culture in the presence of 10 μg/ml AF⁶⁴⁷-OTR4131 at 3, 6, 9, and 12 days. Upper panel: phase contrast; middle panel: Cy5 staining; lower panel: merge (scale bar = 50 μm). e TNC and CD34⁺/CD45⁺ cell ratios between day 0 and day 12 (n = 9 for 0 to 1 μg/ml and n = 4 for 10 and 100 μg/ml OTR4131 concentrations). f TNC and CD34⁺/CD45⁺ cell viability at day 6 (white bars, n = 7) and day 12 (dark bars, n = 5). *p = 0.0024. TNC total nucleated cells