Fig. 1

Alkaline phosphatase staining at day 7. Representative images of basal monoculture controls (a, b, c) and monocultures supplemented with 100 ng/ml vascular endothelial growth factor (VEGF) (d, e, f) of human umbilical vein endothelial cells (HUVEC) (a, d), foetal diaphyseal cells (b, e) and foetal epiphyseal cells (c, f); co-cultures of diaphyseal/HUVEC in basal media (g) and supplemented with 100 ng VEGF (i); co-cultures of epiphyseal/HUVEC in basal media (h) and supplemented with 100 ng VEGF (j). Scale bar 100 μm