Fig. 2

Experimental procedure of bone marrow transplantation in experimental autoimmune encephalomyelitis (EAE) mice. Isolated BM cells from donor mice (C57Bl/6) 6–10 weeks old are cultured and ex vivo transduced with a retroviral vector, which encodes the myelin oligodendrocyte glycoprotein (MOG) gene and green fluorescent protein (GFP) driven by an internal ribosomal entry site (IRES) to assist enumeration and tracking of these cells. Transplanted GFP-expressing cells into irradiated mice following their immunization with MOG_35–55 to induce EAE are assessed in their development or protection of disease, as well as chimerism [20]. LTR long terminal repeat, WT wild type