RETRACTED ARTICLE: Robust method for TALEN-edited correction of pF508del in patient-specific induced pluripotent stem cells

Table 1 PCR conditions for genotyping after nucleofections in repair experiment

‘SHORT PCR’ 351/406 bp DSB target/reverted allele	‘MED PCR’ 1234/1346 bp Homology arms	‘LONG PCR’ 2360/2509 bp Full endogenous/reverted allele
95 5’	95 5’	95 5’
95 30”	95 30”	95 30”
64 15”	64 45”	64 45”
72 30”	72 2’	72 3’
40 cycles	40 cycles	40 cycles
72 7’	72 7’	72 15’

bp Base pair, DSB Double strand break, PCR Polymerase chain reaction. Left numbers on each cell of the table correspond to Temperatures, and right numbers correspond to times for each step of the PCR amplification

ISSN: 1757-6512