SHORT PCR | MED/LONG PCR | ||||
---|---|---|---|---|---|
Working dil | Final conc./rxn | μl/25 μl | Final conc./rxn | μl/25 μl | |
DNA seq-1 696.9 ng/μl | 10−3/10−6 | ≈300 ng | 2.0 μl | ≈300 ng | 2.0 μl |
Primer up | 10 μM | 0.4 μM | 1.0 μl | 0.4 μM | 1.0 μl |
Primer dwn | 10 μM | 0.4 μM | 1.0 μl | 0.4 μM | 1.0 μl |
dNTPs | 2.5 mM | 100 μM | 1.0 μl | 200 μM | 2.0 μl |
Buffer 10× | 10× | 1× | 2.5 μl | 1× | 2.5 μl |
MgCl2 | 50 mM | 3 mM | 1.50 μl | 2 mM | 1.0 μl |
Taq | 5 U/μl | 2.5 U | 0.5 μl | 0.5/1/2.5 U | 0.5 μl |
H2O | 15.5 | 15 |