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Table 2 PCR reaction chemistry

From: RETRACTED ARTICLE: Robust method for TALEN-edited correction of pF508del in patient-specific induced pluripotent stem cells

   SHORT PCR MED/LONG PCR
  Working dil Final conc./rxn μl/25 μl Final conc./rxn μl/25 μl
DNA seq-1 696.9 ng/μl 10−3/10−6 ≈300 ng 2.0 μl ≈300 ng 2.0 μl
Primer up 10 μM 0.4 μM 1.0 μl 0.4 μM 1.0 μl
Primer dwn 10 μM 0.4 μM 1.0 μl 0.4 μM 1.0 μl
dNTPs 2.5 mM 100 μM 1.0 μl 200 μM 2.0 μl
Buffer 10× 10× 2.5 μl 2.5 μl
MgCl2 50 mM 3 mM 1.50 μl 2 mM 1.0 μl
Taq 5 U/μl 2.5 U 0.5 μl 0.5/1/2.5 U 0.5 μl
H2O    15.5   15
  1. PCR Polymerase chain reaction; dil dilution; conc concentration; rxn reaction; dNTPs deoxynucleotides triphosphates mixture; dwn down (3' end)