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Table 2 PCR reaction chemistry

From: RETRACTED ARTICLE: Robust method for TALEN-edited correction of pF508del in patient-specific induced pluripotent stem cells

  

SHORT PCR

MED/LONG PCR

 

Working dil

Final conc./rxn

μl/25 μl

Final conc./rxn

μl/25 μl

DNA seq-1 696.9 ng/μl

10−3/10−6

≈300 ng

2.0 μl

≈300 ng

2.0 μl

Primer up

10 μM

0.4 μM

1.0 μl

0.4 μM

1.0 μl

Primer dwn

10 μM

0.4 μM

1.0 μl

0.4 μM

1.0 μl

dNTPs

2.5 mM

100 μM

1.0 μl

200 μM

2.0 μl

Buffer 10×

10×

2.5 μl

2.5 μl

MgCl2

50 mM

3 mM

1.50 μl

2 mM

1.0 μl

Taq

5 U/μl

2.5 U

0.5 μl

0.5/1/2.5 U

0.5 μl

H2O

  

15.5

 

15

  1. PCR Polymerase chain reaction; dil dilution; conc concentration; rxn reaction; dNTPs deoxynucleotides triphosphates mixture; dwn down (3' end)