Table 3 Sequences of primers used in each step of screening for positive and negative selection steps during edited genome repair
CR4 Up | TAG GTT GTA GCA GTG GTA GTG |
CR4 Down | CTC TAT GTA ACT CTT GTC TCT TCC |
ZFN F | TGG AGC CTT CAG AGG GTA AA |
ZFN R | TTT CAT GTG TTT GCA AGC TTC T |
Integra5.1up | TCC TCT GCT ACC TCC TTT CCT TCT |
Integra5.2up | TCT TCC TCT GCT ACC TCC TTT CCT |
Integra5.3up | TGC TTT TTC TTC CTC TGC TAC CTC C |
Integra5.4up | TTC CTC TGC TAC CTC CTT TCC TTC T |
Integra5.5up | TCC TCT GCT ACC TCC TTT CCT TC |
Integra5.1dn | GGA GCT CCA AGC GGC GAC TG |
Integra5.2dn | GCC TCA CGG GAG CTC CAA GC |
Integra5.3dn | TGA CAA GCA CGC CTC ACG GG |
Integra5.4dn | CTC ACG GGA GCT CCA AGC GG |
Integra5.5dn | ACC GCA TTG ACA AGC ACG CC |
Integra3.1up | ACA GAC CGA TAA AAC ACA TGC GTC A |
Integra3.2up | CAG ACC GATA AAA CAC ATG CGT CA |
Integra3.3up | AGA CCG ATA AAA CAC ATG CGT CA |
Integra3.4up | CAG ACC GAT AAA ACA CAT GCG TCA A |
Integra3.5up | AGA CCG ATA AAA CAC ATG CGT CAA |
Integra3.1dn | AGG CAC CTT CCG CAA CTT TTC CA |
Integra3.2dn | GGC ACC TTC CGC AAC TTT TCC A |
Integra3.3dn | AGG CAC CTT CCG CAA CTT TTC C |
Integra3.4dn | GGC ACC TTC CGC AAC TTT TCC AC |
Integra3.5dn | GGC ACC TTC CGC AAC TTT TCC |
Reint F | CTG CTG CAA CTT ACC TCC GGG ATG |
Reint R | CCA ATC CTC CCC CTT GCT GTC CTG |
Recomb F | TCT GTC ATC AAA GCC TGT CAG C |
Recomb R | GCTCGTTGACCTCCACTCAG |
ENDO F | GCGTCATCAAAGCATGCCAAC |
PB-out-F | GATCTGTCATCAAAGCCTGTCAG |
PB-out-R | CAGATCAATGCTCATTCCATTAGGC |