Table 4 Efficiencies of targeted correction of pF508del in CFhIPS lines
Line | Cell number | Puro | PCR targets | Ratio PCR targets | Efficiency |
|---|---|---|---|---|---|
CFhIPS3.18 | 122 × 106 | 648 | 60 | 9.26 % | 4.9 × 10−5 |
CFhIPS4.4 | 58 × 106 | 98 | 10 | 10.20 % | 1.7 × 10−5 |
Line | Cell number | Puro | PCR targets | Ratio PCR targets | Efficiency |
|---|---|---|---|---|---|
CFhIPS3.18 | 122 × 106 | 648 | 60 | 9.26 % | 4.9 × 10−5 |
CFhIPS4.4 | 58 × 106 | 98 | 10 | 10.20 % | 1.7 × 10−5 |
Stem Cell Research & Therapy