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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: The effects of immunomodulation by macrophage subsets on osteogenesis in vitro

Fig. 4

IL-4 treatment of M1-MC3T3 cocultures at 72 hours enhances osteogenic ability. Untreated and IL-4-treated M1-MC3T3 cocultures were analyzed 2 or 3 weeks after seeding. Week 2 ALP, OC, and OPN gene expression, relative to housekeeping gene 18s and fold change from untreated M1-MC3T3 cocultures, were analyzed by qRT-PCR a–c (left panels). Week 2 ALP activity and week 3 OC and OPN protein secretions were quantified by the p-nitrophenyl phosphate method and ELISA, respectively a–c (right panels). Week 3 cultures were stained with Alizarin Red, destained, and quantified by absorbance at 562 nm d. *p <0.05, **p <0.01, ***p <0.001, and ****p <0.0001. ALP alkaline phosphatase, IL interleukin, OC osteocalcin, OD optical density, OPN osteopontin

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