Fig. 1

hMSCs were cultured in vitro and infected with lentiviral vectors targeting Wnt11. a Passage 0 hMSCs show the typical shape of MSCs. b Representative images of hMSCs with lentivirus transduction. c Quantitative PCR assay of the Wnt11 gene expression level in Wnt11 RNAi-expressing or Wnt1-overexpressing hMSCs after transduction; an empty vector expressing enhanced GFP served as a control. d Western blot assay of the Wnt11 protein expression of Wnt11 RNAi-expressing or Wnt1-overexpressing hMSCs after transduction; an empty vector expressing enhanced GFP served as a control. e Quantitative analysis of the relative protein level of Wnt11; results were based on the density and normalized to β-actin. Data presented as mean ± SD. *p <0.05, **p <0.01 and ***p <0.001 compared with empty vector control. Scale bar = 50 μm