Fig. 2

Exposure to JQ1 caused an increase in MSC size with simultaneous loss of CD105. Cells were treated with 0, 100, and 500 nM JQ1 for 24 hours and 5 days and were subjected to FACs analysis. a Plots displaying size and granularity of cells. Both size and granularity of cells were increased in a concentration and time-dependent manner. b Quantification of percentage of cell populations changed in cell size upon exposure to JQ1. c CD105 loss was proportional to concentration of JQ1. d Quantification of the MSC surface markers of JQ1 upon 5 days of exposure. *p ≤0.05. All plots are representative of three biological experiments. JQ1 (S)-tert-butyl2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetate, MSC mesenchymal stem cell