Fig. 6
From: BET protein inhibitor JQ1 inhibits growth and modulates WNT signaling in mesenchymal stem cells
Effect of JQ1 on apoptosis of MSCs. a Annexin V/FITC and PI staining of MSCs treated with 0, 10, 100 and 500 nM JQ1 for 24 and 72 hours. Plots are representative of the mean number of Annexin V-positive cells (n = 3) from three biological experiments. b qRT-PCR analysis of apoptosis genes including BCL2, BIM, Caspase 8, and Caspase 9 c. *p ≤0.05. c Western blotting showing downregulation of Caspase 8 and Caspase 9 in MSCs treated with 500 nM JQ1. d Caspase 8 and 9 expression was quantified by ImageJ software and normalized to β-actin expression. Ctrl control, FITC fluorescein isothiocyanate, JQ1 (S)-tert-butyl2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetate, PI propidium iodide