Fig. 7

Effect of JQ1 on the expression of pluripotency, WNT signaling, and differentiation genes as determined by qRT-PCR a–d and immunocytochemical analysis e. a Pluripotent markers OCT4, KLF4, Nanog, and SOX2 were downregulated in MSCs treated with 100 nM JQ1 for 24 hours. b WNT signaling genes CTNNB1, AXIN, FZD6, and LRP6 were upregulated and WNT2, FZD2 and FZD4 were downregulated in MSCs treated with 500 nM JQ1 for 24 hours and 5 days. c Osteogenic markers COL1 and RUNX2, chondrogenic markers COL2 and SOX9, and neurogenic marker Nestin were downregulated in MSCs treated with 500 nM JQ1 for 24 hours. d Neuronal markers TUJ1, CCL5, INA and SMURF2 were upregulated in MSCs treated with 500 nM JQ1 for 24 hours and 5 days. e TUJ1 and MAP2 proteins were expressed in MSCs treated with 500 nM JQ1 for 5 days. Images acquired at 10× magnification. f Zoomed-in merged image as shown in e. *p ≤0.05. Ctrl control, DAPI 4′,6-diamidino-2-phenylindole, JQ1 (S)-tert-butyl2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetate