Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Apoptosis and failure of checkpoint kinase 1 activation in human induced pluripotent stem cells under replication stress

Fig. 2

hiPS cells undergo apoptosis and no cell cycle arrest in response to replication inhibitor. a hiPS cell lines MIFF1, MIFF3, and MIFF4 show an increase in the sub-G1 fraction after TdR treatment as reflected by stacked PI profiles obtained by flow cytometry at different time points. hiPS cells show an early accumulation in the S phase but fail to reach G2 phase. b Graph depicting the increasing levels of the sub-G1 fraction determined from their PI profile, according to the time of TdR treatment, for each cell line MIFF1, MIFF3, and MIFF4. c Western blots showing an increased activation of caspase 3 protein level following TdR treatment. Beta-actin is presented as the control. Shef5N, a normal hES cell line, also show this increase in caspase 3 activation, while the somatic cell line HCT116 does not, in response to TdR. d Increased proportions annexinV+/PI– MIFF1 cells, a marker of apoptosis, after TdR treatment. *p <0.05, **p <0.001, ***p <0.0001. MIFF mRNA-induced foreskin fibroblast, PI propidium iodide, TdR thymidine

Back to article page