Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Co-overexpression of TGF-β and SOX9 via rAAV gene transfer modulates the metabolic and chondrogenic activities of human bone marrow-derived mesenchymal stem cells

Fig. 6

Expression analyses in chondrogenically-induced hMSCs upon administration of rAAV vectors. hMSC aggregates were transduced with the various vectors or vector combinations as described in Fig. 1 and in Methods. Samples were processed after 21 days to monitor the gene expression profiles by real-time RT-PCR as described in Methods. The genes analyzed included the transcription factor SOX9, aggrecan (ACAN), type II collagen (COL2A1), type I collagen (COL1A1), and type X collagen (COL10A1), with GAPDH serving as a housekeeping gene and internal control. Threshold cycle (Ct) values were obtained for each target and GAPDH as a control for normalization, and fold inductions (relative to lacZ-treated pellets) were measured using the 2–ΔΔCt method. *,#Statistically significant compared with rAAV-lacZ (*P ≤0.050; # P ≤0.010). TGF-β transforming growth factor beta

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512