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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Intraperitoneally infused human mesenchymal stem cells form aggregates with mouse immune cells and attach to peritoneal organs

Fig. 4

Colocalization of human GFP-MSC with immune cells in the peritoneal cavity. Representative immunofluorescent images of human GFP-MSC in free-floating aggregates, and aggregates attached to the mesentery and omentum 24 h after their IP administration. a In vivo low-magnification (1.5×) images. b GFP-positive aggregates as shown in (a) were obtained from the peritoneal cavity, cryosectioned and stained with CD11b antibody (magnification 20×). c GFP-positive aggregates attached to the mesentery and omentum were isolated with tweezers, fixed, labeled with B220 antibody, and examined as whole mounts (magnification 20×). Representative images from three mice. DAPI 4′,6-Diamidino-2-phenylindole; GFP green fluorescent protein, MSC mesenchymal stem cells

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