Fig. 4

Changes in the T-cell population after coculture with CD146⁺ cells incubated with anti-CD146 in vitro. The splenocytes were cocultured with anti-CD146 antibody-treated CD146⁺ cells or CD146^– cells for 3 days, and the CD4⁺ cell population was measured by flow cytometry a. Changes in the Treg and Th17 cell populations of CD4⁺ cells were analyzed further by flow cytometry b and ELISA c. Data are expressed as mean ± SEM from five independent experiments. *P <0.05, **P <0.01 versus CD146⁺ cells alone. ab antibody, Ctl CD4⁺ T cells, IL interleukin, TH17 T-helper type 17, Treg regulatory T