Fig. 1

Proliferation of p27 overexpressing miPSCs. Overexpression of p27-2A-mRFP in transient transfected miPSCs are shown with mRFP fluorescence (a) and immunostaining of p27 (b) with nuclear staining by DAPI. The bar represents 100 μm. c, d Time lapse images of phase contrast (c) and mRFP (d) in p27-2A-mRFP transfected miPSCs. e Relationship between the number of cell divisions and intensities of mRFP in p27-2A-mRFP expressing miPSCs (closed circles). An open circle shows the average of the number of cell divisions in control miPSCs (n = 3). Dotted line shows fitting line of results of p27-2A-mRFP expressing cells with exponential function. f Phase contrast (left) and mRFP fluorescent (right) images of miPSCs-p27. g Proliferation rate of miPSCs (closed circles) and miPSCs-p27 (open circles). h Comparison of the percentages of trypan blue stained cells between miPSCs and miPSCs-p27. Error bars correspond to the SEM (n = 3). *P < 0.05, student’s t-test. mRFP monomeric red fluorescent protein