Fig. 3

Differentiation of miPSCs-p27. a–d Images of EBs from miPSCs and miPSCs-p27 on the sixth day of EB formation. Fluorescent images of miPSCs and miPSCs-p27 are shown in (a) and (b), respectively. Phase contrast images of miPSCs and miPSCs-p27 are shown in (c) and (d), respectively. e–h Images of beating EBs from miPSCs and miPSCs-p27. Fluorescent images of miPSCs and miPSCs-p27 are shown in (e) and (f), respectively. Phase contrast images of miPSCs and miPSCs-p27 are shown in (g) and (h), respectively. i, j Expression of GATA-4, cTnT, and Mef2c after EB formation is shown. RT-PCR analysis of the genes (i) and relative intensities of the mRNA to GAPDH expression (j) of EBs from miPSCs and miPSCs-p27 are shown. High expressions of the genes were detected in beating EBs derived from miPSCs or miPSCs-p27. *P < 0.05, comparison to 0 days of miPSCs with Tukey-Kramer test. k, l Expression of pluripotent marker genes are shown. RT-PCR analysis of stem cell marker genes (k) and relative intensities (l) of miPSCs and miPSCs-p27 are shown. *P < 0.05, student’s t-test. Error bars correspond to the SEM (n = 3). m, n Protein expression of pluripotent marker genes are shown. Western blots of stem cell marker genes (m) and relative intensities (n) of miPSCs and miPSCs-p27 are shown. *P < 0.05, student’s t-test. Error bars correspond to the SEM (n = 3). EB embryoid body, miPSC mouse induced pluripotent stem cell, miPSCs-p27 p27 overexpressing mouse induced pluripotent stem cells