Fig. 4

Teratoma formation of p27 expressing pluripotent stem cells. a–f Representative images of sections from miPSCs or miPSCs-p27 derived teratomas. Hematoxylin and eosin staining of teratoma derived from miPSCs (a–c) and miPSCs-P27 (d–f). Teratomas contained tissues derived from all three germ layers. g, h Teratomas were formed by subcutaneous injection of miPSCs (g) and miPSCs-p27 (h) into nude mice. i, j Teratomas from miPSCs (i) and miPSCs-p27 (j) injected nude mice. k Average weight of teratomas from miPSC and miPSC-p27 injected nude mice. l, m Expression of pluripotent marker genes are shown. RT-PCR analysis of stem cell marker genes (l) and relative intensities (m) of miPSCs and miPSCs-p27 are shown. Error bars correspond to the SEM (n = 3). *P < 0.05, student’s t-test. miPSC mouse induced pluripotent stem cell, miPSCs-p27 p27 overexpressing mouse induced pluripotent stem cells