Fig. 1

Characteristics of USCs and USCs-Exo. a) The morphology and growth of USCs. Scale bar = 200 μm. b) USCs were characterized by flow cytometry using the surface markers CD29, CD90, CD73, CD44, CD34, CD45 and HLA-DR. White solid peaks represent the isotype controls and the grey solid peak represents the marker indicated. c) Morphology of USCs-Exo under a transmission electron microscopy. d) TRPS measurement showed that the size range of USCs-Exo concentrated at 50–100 nm, and the measured mean concentration (particles/ml) of USCs-Exo was 5.2E + 009. e) Western blotting analysis of exosome-specific CD9, CD63 and CD81 proteins in USCs and USCs-Exo. USC Urine-derived stem cell, USCs-Exo Exosome from urine-derived stem cells