Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Conditioned umbilical cord tissue provides a natural three-dimensional storage compartment as in vitro stem cell niche for human mesenchymal stroma/stem cells

Fig. 1

Morphology and cell cycle properties of recultured UC tissue. a Cryopreserved pure UC290115 tissue pieces in liquid nitrogen without cryobuffer or any other additives (upper panel) and in the presence of cryobuffer (lower panel) were recultured in MSC medium for 14 days and the morphology of potential MSC was documented (magnification bars = 100 μm). b Potential MSC from 14-day explant cultures of recultured and previously cryopreserved UC290115 tissue pieces in liquid nitrogen without cryobuffer (upper panel) and in the presence of cryobuffer (lower panel) were harvested and subjected to cell cycle analysis. Distribution within sub-G1, G0/G1, S, and G2/M cell cycle phases was performed by FloMax cell cycle software. c Reculture of conditioned UC100314re(1) tissue pieces after cryopreservation in liquid nitrogen with cryobuffer revealed the outgrowth of MSC-like cells (MSC100314UC-re(1)) within 8 days (upper panel). In addition, a second reculture after repeated cryopreservation of these UC tissue pieces (UC100314 re(2)) demonstrated an explant culture of MSC-like cells (MSC100314 UC-re(2)) within 14 days (magnification bars = 100 μm). MSC mesenchymal stroma/stem cells

Back to article page