Fig. 3

Cell yield and surface marker analysis of explants from GFP-labeled UC tissues. a Quantification of total cell number and GFP-positive cells after explant culture of GFP-labeled UC tissue was performed by flow cytometry (upper panel). The outgrowing cells after 21 days were analyzed for the PE-labeled MSC-specific markers CD73, CD90, and CD105, demonstrating the amount of PE-positive unlabeled cells and the appropriate amount of antibody-positive and GFP-positive cells within the double fluorescence channel, respectively (lower panel). b Comparison of the amount of outgrowing cells with the MSC-like characteristic surface marker expression CD73, CD90, and CD105 from fresh (upper panel) and conditioned (lower panel) UC tissue was quantified after 13 days of explant culture and calculated as cell yield per milligram of tissue. FACS fluorescence-activated cell sorting, FITC fluorescein isothiocyanate, GFP green fluorescent protein, PE phycoerythrin