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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Conditioned umbilical cord tissue provides a natural three-dimensional storage compartment as in vitro stem cell niche for human mesenchymal stroma/stem cells

Fig. 5

MSC surface marker analysis. Cell surface marker evaluation was performed for the presence of the typical MSC markers (CD73, CD90, and CD105) by flow cytometry analysis in cells of three individual UC explant cultures of the previously cryopreserved UC tissues. a MSC100314UC-re(1). b MSC110314UC-re(1). c MSC180314UC-re(1). Moreover, the MSC marker expression was compared with cells obtained after second reculture following repeated cryopreservation of these UC tissue pieces. d MSC100314UC-re(2). e MSC110314UC-re(2). f MSC180314UC-re(2). A simultaneous absence of further cell type-specific surface markers CD31, CD34, CD45, and CD14 was tested. The analysis was performed in steady state-growing cells by flow cytometry compared with the corresponding immunoglobulin isotype antibodies which were used as appropriate controls in the histograms. MSC mesenchymal stroma/stem cells

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