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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair

Fig. 1

Generation of hiPSCs from peripheral blood. a Phase-contrast image of the PBCs. Scale bar = 100 μm. b Phase-contrast image of a hiPSC line. Scale bar = 100 μm. c Karyotype (46,XY). d Fluorescent immunostaining of pluripotent markers OCT4, SSEA4, TRA-1-60, NANOG, and TRA-1-81. Scale bars = 100 μm. e Teratoma formation. Teratomas were harvested over 2 months after subcutaneous injection of the iPSCs into SCID mice. (i) Neuroepithelial cells, (ii) cartilage, and (iii) respiratory epithelium were detected by hematoxylin and eosin staining. Scale bar = 50 μm

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