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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair

Fig. 4

Characterization of hiPSC–MSC chondrogenic pellets. a Alcian blue staining and b toluidine blue staining of glycosaminoglycans and proteoglycans revealed the chondrocyte-type appearance of the hiPSC–MSC chondrogenic pellets. Scale bars = 100 μm. c, d Immunohistochemistry for type II and type X collagen. Scale bars = 100 μm. e Biochemical characterization of hiPSC–MSC chondrogenic pellets (hiPSC-Chon) versus hiPSCs, EBs, hiPSC–MSC-like cells (hiPSC–MSCs) and hMSC chondrogenic pellets (hMSC-Chon) versus hMSCs. sGAG per DNA. Bars represent means ± SEM; n = 3; *P < 0.05. EB embryoid body, hiPSC human induced pluripotent stem cell, hMSC human mesenchymal stem cell, sGAG sulfated glycosaminoglycan

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Stem Cell Research & Therapy

ISSN: 1757-6512