Fig. 4

Quantification of microglial cells in the anterior horn of the spinal cord. The number of Iba1-positive cells was quantified in week 12 in the presymptomatic injected animals b–d and in week 15 in the symptomatic injected animals e–g. Iba1-positive cells in the wild-type animals are shown in a. The saline-injected and BMMC-injected mice showed an increase in the number of Iba1-positive cells compared with the wild-type mice. There was no difference in the number of microglia cells after the treatment with BMMC injected in week 9 or week 14. *p <0.01, **p <0.001. Scale bar: 50 μm. BMMC bone marrow-derived mononuclear cells, SOD1 Cu/Zn superoxide dismutase 1