Fig. 6
Fate tracing of BMMC-injected cells stained with CellTrace™. BMMC stained with the fluorescent cell marker CellTrace™ (green) were transplanted into the spinal cord of SOD1G93A mice in week 14. Photomontage of confocal images showing a transverse section of lumbar spinal cord 7 days after BMMC transplant. We observed a dispersed pattern of CellTrace™ staining in the ventral horn. Microglia were stained with Iba1 (red). Observe the high concentration of microglia at the injection site (white box in a). b–d Higher magnification of the area delimited by the white box in a, showing the association of BMMC with microglia stained with Iba1. Arrows in b–d indicate microglia phagocyte profiles interacting with the transplanted cells. Scale bar: 50 μm