Skip to main content
Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Intraspinal bone-marrow cell therapy at pre- and symptomatic phases in a mouse model of amyotrophic lateral sclerosis

Fig. 7

In vivo fate tracing of BMMC-injected cells. BMMC were labeled with SPION and injected into the lumbar spinal cord in week 9 a–c or in week 14 d–f. Representative images of in vivo MRI in longitudinal planes of the spinal cord at different time points. Arrows indicate hypointense (black) areas corresponding to SPION-labeled cells in the spinal cord. Labeled cells were found at day 1 a, d, day 8 b, e, and at the end stage of the disease, which corresponds to 72 days after presymptomatic injection c and 34 days after symptomatic injection f. g Transverse section of the spinal cord from the animal analyzed by MRI in f. SPION staining is revealed by differential and interference contrast (dark signal in g–i). Iba1 was used to identify microglia/macrophages (red). We observed only faint staining throughout the spinal cord, with most of the SPION staining located in the ventral horn. Some SPION staining was associated with Iba1-positive cells (arrowheads in i) and some with Iba1-negative cells (arrows in i). Scale bar: 50 μm

Back to article page