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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment

Fig. 5

Differentiation potential of MSCs from equine SF-H, SF-OCD, SF-OA, SM-H, SM-OCD, and SM-OA. Differentiation of MSCs from SF and SM into mesenchymal lineages during P3. MSCs after chondrogenic differentiation stained with toluidine blue showing hyaline matrix (blue): SF-H a, SF-OCD b, SF-OA c, SM-H d, SM-OCD e, and SM-OA f (400× magnification). MSCs stained with Alizarin Red showing matrix calcium formation, confirming the osteogenic lineage (red): SF-H g, SF-OCD h, SF-OA i, SM-H j, SM-OCD k, and SM-OA l. MSCs showing intracytoplasmic lipid droplets confirming the adipogenic lineage: SF-H m, SF-OCD n, SF-OA o, SM-H p, SM-OCD q, and SM-OA r (1000×). Chondrogenic differentiation area s. Osteogenic differentiation area t. Quantification of positive matrix area was performed using AxioVision LE64 software (Carl Zeiss). The program analyzed 10 photographs of each plate (magnification = 10×) and calculated the area of positive matrix when blue and red stains for chondrogenic and osteogenic differentiation, respectively, were observed. *P ˂0.05. SF-H synovial fluid from healthy joints, SF-OA synovial fluid from joints with osteoarthritis, SF-OCD synovial fluid from joints with osteochondritis dissecans, SM-H synovial membrane from healthy joints, SM-OA synovial membrane from joints with osteoarthritis, SM-OCD synovial membrane from joints with osteochondritis dissecans (Color figure online)AU Query: Confirm Fig 5 caption after editing to style OK

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