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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Hypoxic conditioned medium from mesenchymal stem cells promotes lymphangiogenesis by regulation of mitochondrial-related proteins

Fig. 1

hMSC-derived HCM stimulate hLECs in vitro. a hLEC proliferation was measured using the Cell Counting Kit-8 assay. b Representative images showed the results of migration assays of hLECs treated with NCM and HCM. c Representative photographs of tube formation in hLECs after treatment with NCM and HCM. d hLEC specific marker, LYVE-1, and phosphorylation of ERK were detected using western blot analysis. Values are represented as the mean of three measurements with the SD indicated by error bars. Control: EBM. *p <0.001, # p <0.05. ERK extracellular signal regulated kinase, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HCM hypoxic conditioned media, LYVE-1 lymphatic vessel endothelial hyaluronan receptor 1, NCM normoxic conditioned media

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