Fig. 3

Effects of HCM on expression of mitochondria-related genes in hLECs. a hLECs were starved in EBM for 12 hours and then subjected to hMSC-conditioned medium treatment for 12 hours. MFN1 and MFN2 mRNA expression were analyzed using RT-PCR. mRNA expression was normalized using GAPDH. b hLECs were starved in EBM for 12 hours and then subjected to hMSC-conditioned medium treatment for 12 hours. MFN1 and MFN2 expression was measured using western blot analysis. c Expression of MFN1/2 was detected in MFN1/2 siRNA-transfected hLECs and the expression of MFN1/2 by immunoblotting. d Representative photographs were tube formation of MFN1/2 siRNA-transfected hLECs after treatment of each media. Control: EBM. Values are represented as the mean of three measurements with the SD indicated by error bars. *p <0.001, ^# p <0.05. GAPDH glyceraldehyde 3-phosphate dehydrogenase, HCM hypoxic conditioned media, MFN mitofusin, NCM normoxic conditioned media, siRNA small interfering RNA