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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Genome-wide gene expression analyses reveal unique cellular characteristics related to the amenability of HPC/HSCs into high-quality induced pluripotent stem cells

Fig. 1

Transcription factor-mediated reprogramming of HPC/HSCs results in high-quality iPSCs with full pluripotency. a Schematic showing the generation of iPSCs from HPC/HSCs. b Flow cytometry analysis of the purity of HPC/HSCs isolated by MACS. First column, cells were isolated based on size, as indicated by side scatter (SSC) and forward scatter (FSC). Second column, CD45-positive cells were further selected. Third column, c-Kit and CD45 double-positive cells were isolated. c The reprogramming efficiency of HPC/HSCs was analyzed using AP staining. MEFs were used as the control (n = 3 measurements). Error bars indicate the SD. **P <0.01, unpaired t test. d Viable 4N mice and the offspring (F1) of 4N mice derived from HPC/HSC-iPSCs with the indicated cell line. e Genetic characterization of the 4N mice using SSLP analysis. f Summary of the derivation of mice from HPC/HSC-iPSCs. #Karyotype was considered normal when greater than 80 %. Dox doxycycline, E19.5 embryonic day 19.5, HPC/HSC hematopoietic progenitor and stem cell, iPSC induced pluripotent stem cell, MEF mouse embryonic fibroblast, 2N chimera, 4N tetraploid complementation, ND not determined, OSKM Oct4, Sox2, Klf4, and c-Myc. APC Allophycocyanin, FITC Fluorescein isothiocyanate

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