Fig. 5

Improvements in PSC purity. a Optimization of the PSC isolation process has led to increased purity of cells, as indicated by enrichment for the adventitial cell antigen CD34 (left), and the dramatic reduction in the endothelial cell antigen CD31 (middle) and haematopoietic marker CD45 (right) by real-time quantitative PCR (n = 7; pre optimization = 4, post optimization = 3). b (Left) FSC vs SSC demonstrating the population of lymphocytes (arrow). (Centre) Confirmation of lymphocytes by demonstration of CD45⁺ phenotype of the subpopulation. (Right) Selection of the CD45^– depleted cellular fraction for subsequent analysis. FSC Forward scatter, PSC perivascular stem cell, SSC side scatter