Fig. 4

Decidua-derived mesenchymal stem cell (DMSC) administration to healthy mice reduces IL-17 production. Healthy C57BL/6 mice (5 mice/group) were exposed to three successive doses of DMSCs (DMSC group) or PBS (Naïve group) at 3-day intervals. Seven days after the first DMSC inoculation, total spleen cells from each group were pooled and stimulated in vitro by anti-CD3/anti-CD28. Each sample was assayed in quintuplicate. Results are shown from one representative out of three independent experiments. Proliferation, soluble IL-17 released to the medium, and IL-17 mRNA were measured by the MTT colorimetric method, ELISA and RT-qPCR, respectively. Significance was analyzed by t-test; standard error of the means are shown. **p < 0.01, ***p < 0.001. IL Interleukin, OD Optical density