Fig. 7

Decidua-derived mesenchymal stem cell (DMSC) treatment of experimental autoimmune encephalomyelitis (EAE) modifies the cell composition of inflammatory infiltrates in the CNS. CNS infiltrates from C57BL/6 EAE and DMSC-treated mice (n = 4/group) were obtained at day 20 p.i. and subjected to flow cytometry analysis. Debris and doublets were excluded and live/dead discrimination was determined using propidium iodide. Percentages of CD4⁺IL-17⁺ were obtained by analysis of surface and intracellular staining with anti-CD4 and anti-IL17, respectively (a). CD11b⁺ Lineage^- cells (gated out using CD4, CD8, CD19, CD11c, NK1.1 biotin-PE-Cy7 dump channel) were then subgated for identification of CD11b⁺Ly6G⁺ subpopulation (infiltrating neutrophils) (b) or CD11b⁺Ly6C⁺SSC^low subpopulation (infiltrating monocytes) (c) cells are shown by representative flow dot plots from two experiments. Data for quantification of infiltrating cell types in DMSC-treated mice are shown as percentages of each subset found in untreated mice (d). Significance was analyzed by t-test; standard error of the means are shown. *p < 0.05. IL Interleukin