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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study

Fig. 1

Cell culture characterization. a Human UC-MSC immunophenotype, positive for CD73, CD90, and CD105 and negative for CD45, CD34, CD11b, CD19, and HLA-DR. b Multilineage differentiation of UC-MSCs. Differentiation into adipocytes was revealed by Sudan III staining for intracellular accumulated lipids. Differentiation into osteocytes was revealed by Alizarin Red S staining for calcium mineralization. Chondrogenic differentiation was revealed by Alcian blue staining for mucopolysaccharides. Scale bar 100 μm. c Positive staining of EA.hy926 cells for CD31 as endothelial marker. Scale bar 100 μm. d Concentration of soluble forms of VEGF-A (VEGF-A-121 and VEGF-A-165) in the EA.hy926-conditioned media and the UC-MSC-conditioned media. Values are expressed as average ± SD of three replicates. *p <0.05. h hours, UC-MSC umbilical cord-derived mesenchymal stromal/stem cell, VEGF vascular endothelial growth factor

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