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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study

Fig. 5

Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31+ cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs (red). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 (green). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)

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Stem Cell Research & Therapy

ISSN: 1757-6512