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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Minicircle DNA-mediated endothelial nitric oxide synthase gene transfer enhances angiogenic responses of bone marrow-derived mesenchymal stem cells

Fig. 2

Gene delivery efficiency of P-GFP and MC-GFP DNA vectors. a Gel electrophoresis of P-GFP and MC-GFP plasmids following plasmid purification and enzyme digestion. b Fluorescence microscopy of transfected HEK293T cells and rBMSCs with P-GFP and MC-GFP with a range of plasmid concentrations and quantitation by flow cytometry of transfection efficiencies for (c) HEK293T cells and (d) rBMSCs. MC-GFP minicircle vector expressing green fluorescent protein, P-GFP plasmid vector expressing green fluorescent protein, rBMSC rat bone marrow-derived mesenchymal stem cell

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