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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Minicircle DNA-mediated endothelial nitric oxide synthase gene transfer enhances angiogenic responses of bone marrow-derived mesenchymal stem cells

Fig. 5

Expression of eNOS and NO production in transfected rBMSCs. a Fluorescence microscopy of transfected of rBMSCs with P-eNOS and MC-eNOS with 0.5 μg plasmid DNA. b Transfection efficiency of MC-eNOS and P-eNOS. c Detection of eNOS protein expression in transfected rBMSC by western blot analysis. d NO production in rBMSC cells at 24 hand 48 h post-transfection with P-eNOS and MC-eNOS plasmids using the griess assay. e Detection of nitric oxide production in living cells following P-eNOS and MC-eNOS transfection by DAF-2 fluorescence. # p < 0.05 vs. P-eNOS; *p < 0.05 and **p < 0.05 vs. MC-GFP, P-GFP, and rBMSC. MC-eNOS minicircle vector expressing endothelial nitric oxide synthase, MC-GFP minicircle vector expressing green fluorescent protein, P-eNOS plasmid vector expressing endothelial nitric oxide synthase, P-GFP plasmid vector expressing green fluorescent protein, rBMSC rat bone marrow-derived mesenchymal stem cell

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Stem Cell Research & Therapy

ISSN: 1757-6512