Fig. 7
In vitro cell scratch assay of eNOS transfected rBMSCs and HEK293T cells. Phase-contrast microscopy images of transfected and control cell migration at (a) 0 and 1 h for rBMSCs and (b) 0 and 17 h for HEK293T post-cell scratch and effect of the NO inhibitor L-NAME. Percentage of cell migration (c) at 1 h for rBMSCs and (d) 17 h for HEK293T post-cell scratch and effect of the NO inhibitor L-NAME. *p < 0.05 and **p < 0.05 vs. MC-eNOS (L-NAME), P-eNOS (L-NAME), MC-GFP, P-GFP, rBMSC and HEK293T. L-NAME, L-NG-nitroarginine methyl ester, MC-eNOS minicircle vector expressing endothelial nitric oxide synthase, MC-GFP minicircle vector expressing green fluorescent protein, P-eNOS plasmid vector expressing endothelial nitric oxide synthase, P-GFP plasmid vector expressing green fluorescent protein, rBMSC rat bone marrow-derived mesenchymal stem cell