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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Delineating the effects of 5-fluorouracil and follicle-stimulating hormone on mouse bone marrow stem/progenitor cells

Fig. 4

Mouse BM stem/progenitor cells survive and proliferate in response to 5-FU treatment. a Flow cytometry analysis of LIN/CD45 VSELs and LIN/CD45+ HSCs showed an increase in percentage post treatment compared with control. Compared with D2, a significant increase was observed on D4. On D10 the percentage of both these cells is reduced (N = 12, ***p ≤ 0.001). b Propidium iodide-based cell cycle analysis of BM cells carried out on D2, D4, and D10 after 5-FU compared with the control showed a decrease in S-phase cells on D2 and an increase in the G0/G1 cell percentage. By D4 the percentage of S-phase cells increased and continued until D10. The increase in S-phase cells suggests proliferation of cells in response to 5-FU treatment (N = 6, ***p ≤ 0.001). c Proliferation of VSELs (LIN/CD45) and HSCs (Lin/CD45+) was confirmed by an increase in percentage of BrdU-positive cells on D4 compared with control (N = 5, **p ≤ 0.01,***p ≤ 0.001). In (a-c) Bars represent average ± SD. Day 2-FU, Day 4-FU, Day 10-FU days post 5-fluorouracil treatment, BM bone marrow, VSEL very small embryonic-like stem cell, HSC hematopoietic stem cell, BrdU bromodeoxyuridine

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