Fig. 1

Relationship between the long-term risk of coronary heart disease events and the regenerative performance of EDCs. a Representative images of HUVEC tubules formed after exposure to media conditioned by EDCs sourced from patients with variable LTS scores. Correlation between patient LTS score and the ability of EDC conditioned media to stimulate HUVEC tubule formation (n = 13). b Representative images of 4′,6-diamidino-2-phenylindole-stained circulating angiogenic ells (CACs) after 24 hours exposure to media conditioned by EDCs sourced from patients with variable LTS scores. Correlation between patient LTS score and the ability of EDC conditioned media to recruit CACs across a transwell membrane (n = 11). c Correlation between patient LTS score improvements in echocardiographic left ventricular ejection fraction (LVEF) measured 2 and 3 weeks after intramyocardial injection into an immunodeficient mouse model of myocardial ischemia (n = 37). d Correlation between LTS score and cytokine content within EDC conditioned media (IL-6, interleukin 6, n = 13; SDF-1α, stromal-derived factor 1 alpha; n = 15). e Representative screen capture images of media conditioned by EDCs from patients with high and low LTS score (white arrows indicate exosomes). Correlation between LTS score and number of exosomes within EDC conditioned media (n = 8). Also shown adjacent to each point is the mode ± SEM size distribution (n = 3 technical repeats per sample). EDCs explant derived cells, HUVEC human umbilical vein endothelial cell, LTS long term stratification