Fig. 6

Lin28A over-expression has various effects on mRNA transcription. Real-time PCR analysis revealed over-expression of Lin28A increased hexokinase II mRNA expression (a). No significant differences were observed in expression levels of HIF1α (b), PDK2 (c) or citrate synthase (d). Mitophagy markers PINK1 and MFN2 were significantly increased in Lin28A over-expressing cells (e and f). 18S was used as a housekeeper gene for normalisation purposes. Student’s unpaired t-tests were used to compare between experimental groups. Data are represented as mean ± SEM, n = 3 for each group. HIF1α hypoxia inducible factor 1 α, PDK2 pyruvate dehydrogenase kinase 2, PINK1 PTEN-induced kinase1, MFN2 mitofusin 2, CS citrate synthase