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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Relevance of HCN2-expressing human mesenchymal stem cells for the generation of biological pacemakers

Fig. 2

Measurement of apoptosis in transfected and non-transfected hMSCs. a Representative profiles of cell population (left) and apoptosis (right) of mHCN2-transfected cells. b Quantitative viability and apoptosis of non-transfected hMSCs (negative control), pIRES-EGFP (pIRES control) and pIRES-mHCN2-EGFP (mHCN2-transfected cells; Hcn2-expressing) transfected hMSCs. Data are presented as means ± SD from three independent transfection experiments. Transfected cells were investigated by Muse® equipment 5 days after their growth with 50 μM geneticin. Non-transfected cells grew and were investigated in parallel. The induction of apoptosis in transfected compared to non-transfected cells was statistically insignificant

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