Fig. 6

Growth of mHCN2-expressing cells on Kapton® scaffold. a Light microscopy image of mHCN2 cells grown on the Kapton® scaffold. Magnification 20×. b Fluorescent image of mHCN2-transfected hMSC grown on the Kapton® scaffold. Magnification 20×. c Confocal three-dimensional image of mHCN2-transfected cells (green). Magnification 20×. Scale bar 10 μm. d Confocal three-dimensional image of mHCN2-transfected cells (green) and nucleus (blue). Magnification 20×. Scale bar 10 μm. e Confocal three-dimensional image of non-transfected hMSCs stained with PKH26 (red) and nucleus (blue). Magnification 20×. f Confocal three-dimensional image of mHCN2-transfected cells (green) on the one side of Kapton® scaffold and hMSC stained with PKH26 (red) on the opposite side. Nuclei were stained blue. Magnification 20×