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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Chm-1 gene-modified bone marrow mesenchymal stem cells maintain the chondrogenic phenotype of tissue-engineered cartilage

Fig. 1

a RT-PCR evaluation of Chm-1, Col II, and AGG gene expression in chondrocytes, MSCs, and T-MSCs cultured in vitro. GAPDH was used as an internal control. Intensity levels showing Chm-1, Col II, and AGG mRNA expression levels in T-MSCs and chondrocytes were significantly higher than those of MSCs (**P < 0.01, mean ± SD, n = 4). b Western blot assay for Chm-1, Col II, and AGG expression. Chm-1, Col II, and AGG expression were significantly upregulated in T-MSCs and chondrocytes. Analysis of band intensities indicated that Chm-1, Col II, and AGG expression levels higher in T-MSCs and chondrocytes than the levels in MSCs (**P < 0.01, mean ± SD, n = 4). c Immunofluorescence examination showed cell transduction efficiency 5 days after transduction. Chm-1 and Col II protein expression was evaluated by immunofluorescence examination. Data are represented as mean ± standard deviation from four independent sets of experiments. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001  BMSC bone marrow mesenchymal stem cell, Chm-1 Chondromodulin-1, T-BMSC Ad5-Chm-1 transfected BMSC

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