Fig. 4

Injection of hMSCs does not prevent OA development or progression. Following two consecutive injections of 1 U collagenase over 2 days (1 U per day), mice were injected with untransduced, AdNull-transduced or AdIL-10-transduced hMSCs (or AdIL-10 virus or vehicle as control) at 1 week post OA induction. After 6 weeks knees were harvested and analysed for cartilage damage and synovial hyperplasia. a, b Median damage scores in the medial and lateral femur (maximum score of 6 with three blinded observers, approximately six sections per knee). c, d Median damage scores in the medial and lateral tibia compartments (maximum score of 6 with three blinded observers, approximately six sections per knee). e Total damage within the four joint compartments (maximum score of 24). f, g Median scores of synovial hyperplasia in the medial and lateral synovial compartments (maximum score of 3 with three blinded observers, approximately six sections per knee). Mild OA was observed in all groups with no significant reduction in the treated groups. Overall there appeared to be more damage in the medial compartment. n = 8 animals per group. MSC mesenchymal stem cell, OA osteoarthritis