Fig. 3From: Development of a two-step protocol for culture expansion of human annulus fibrosus cells with TGF-β1 and FGF-2Cell numbers in the five groups. Approximately 3 × 104 hAF cells were placed in each P60 dish and cultured. Cells were harvested and counted at days 3, 7, 10, and 14. The results were averaged and expressed as the mean ± standard deviation. Each point indicates the mean of three experiments in the same group (n = 6). *Cell numbers were significantly greater in groups 4 and 5 than in groups 1, 2, and 3 at 7 and 10 days of cultivation. **Cell numbers in groups 3 and 4 were significantly greater than in group 5 at 14 days of cultivation. FGF fibroblast growth factor, GF growth factor, TGF transforming growth factorBack to article page