Fig. 4

Relative expression of the BrdU results in the five groups normalized by the control group. hAF cells (1500) were placed in each well of a 96-well plate for the five groups. Cell proliferation was evaluated by luminometer. Each point indicates the mean of three experiments in the same group (n = 6). The results of the relative expression ratio in the different groups were normalized by the control group at different days of cultivation. The relative expression ratio was expressed as the mean ± standard deviation. At days 3, 7 and 10 of cultivation, groups 4 and 5 had significantly faster cell proliferation rates than groups 1, 2, and 3. The group 4 had the fastest cell proliferation rate at 14 days of cultivation. BrdU 5-bromo-2’-deoxyuridine, FGF fibroblast growth factor, GF growth factor, TGF transforming growth factor