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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: EDTA soluble chemical components and the conditioned medium from mobilized dental pulp stem cells contain an inductive microenvironment, promoting cell proliferation, migration, and odontoblastic differentiation

Fig. 2

Characterization of regenerated tissue after extracted tooth transplantation. Twenty-eight days after transplantation of (a, e, j, n) nonextracted tooth, (b, f, k, o) HCl-extracted tooth, (c, g, l, p) GdnHCl-extracted tooth, and (d, h, m, q) EDTA-extracted tooth. a–d In-situ hybridization analysis of mRNA expression of thyrotropin-releasing hormone degrading enzyme (TRH-DE) as a pulp marker. e–h In-situ hybridization analysis of enamelysin as an odontoblast marker using an anti-sense probe reactive to both porcine and mouse genes. Odontoblastic processes (arrows) extending into the tubular dentin. i Comparison of the numbers of enamelysin-positive cells along the dentinal wall. j–m Immunostaining with periodontal ligament-associated protein 1 (PLAP-1, green) merged with Hoechst 33342 (blue). n–q Immunostaining with periostin (green) merged with Hoechst 33342 (blue). Typical positive cells (arrows). r Ratio of the PLAP-1-positive cell number to the total cell number. Data are expressed as means ± SD of four determinations. *P < 0.05, **P < 0.01 (Color figure online)

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Stem Cell Research & Therapy

ISSN: 1757-6512